Yunnan University YU HONG Professor Cordyceps Research Team conducts investigation and research training on Cordyceps biological resources at Royal Thai Polytechnic University


Figure 1. Investigation of Cordyceps in the Khun Tan District of Chiang Rai, Thailand

From May 22 to May 29, 2019, at the invitation of the Royal Thai Polytechnic University, Professor YU HONG and PhD student Wang Dong of the Yunnan University of China (Yunnan)-Southeast Asian Cordyceps Biological Resources Sustainable Utilization Center to the Royal Institute of Technology, Thailand Conduct an academic visit. Rajamangala University of Technology Thanyaburi is one of the member units of the China (Yunnan)-Southeast Asian Cordyceps Biological Resources Sustainable Utilization International Joint Research Center. The purpose of this trip is to conduct a survey of Cordyceps for researchers at the Thai Royal Institute of Technology. , collection, specimen production, strain separation, artificial culture and other skills training, the Thai Cordyceps biological resources investigation and research and the Royal Thai Institute of Technology deepening cooperation, set up a joint investigation team to investigate the biological resources of Cordyceps sinensis in Thailand.

Located in the central part of the Central South Peninsula, Thailand is the center of Southeast Asia. It is located in the tropics. Most of the areas are tropical monsoon climates. The annual average temperature is 24~30°C, the annual temperature is below 18°C, and the average annual precipitation is about 1000mm. Vegetation types and insect species. Due to its unique geographical location, climatic conditions and extremely rich flora and fauna resources, Thailand has become one of the hotspots of biodiversity research. Due to Thailand's superior ecological environment, it is rich in Cordyceps biological resources. Some world-famous scholars such as Jaent Jennifer Luangsa-ard and Nigel Hywel-Jones have done a lot of research on Cordyceps resources in Thailand. The resources are rich and diverse, and it is one of the centers of tropical Cordyceps diversity. So far, the investigation and research on the biological resources of Cordyceps in Thailand is not comprehensive and in-depth. Therefore, the investigation and research on the biological resources of Cordyceps in Thailand is conducive to further comprehensive understanding of the biological resources of Cordyceps in Thailand and the sustainable use of the biological resources of Cordyceps in Thailand, which is of great significance to the regional economic development of Thailand.

The Royal Thai Polytechnic University is a comprehensive public university of science with a dual function of higher education and academic research in Thailand. On June 11, 2018, Dr. Prasert Pinpathomrat, President of Royal Institute of Technology, visited the Yunbaicao Laboratory of Yunnan University, and signed a cooperation with Yunnan University to jointly build a joint study on the sustainable use of Cordyceps biological resources in China (Yunnan)-Southeast Asia. Center's agreement.

During the academic visit of the Royal Thai Polytechnic University, researchers at the Royal Thai Polytechnic University of Thailand conducted training on the investigation, collection, specimen preparation, strain separation, and artificial culture of the Royal Thai Institute of Technology, and conducted investigations on Cordyceps biological resources in the Chiang Rai area. as follows:

1. Royal Thai Academy of Sciences Cordyceps Research Training Course

At 8:30 on May 23 morning, Yunnan University professor YU HONG Cordyceps research team received a warm welcome at the Royal Institute of Technology in Thailand, to attend the opening ceremony of staff Dr. Laliata Siriwattananon president of the Thai Medical College, Dr. Preeyanat Hongthong, Dr. Pacharathon Simking, Surachai Techaoei Dr. Panart Thongpoem, Dr. Phraomas Charoenrak, Dr. Master Khemjira Jarmkom, rosarin Master Taksin, Master Thisakorn Dumrongphuttidecha, Master Oam Sthityanak, Master Sinsamut SaeNgow, master's and other Narakamon Khamwaree. Dr. Laliata Siriwattananon, Dean of the Thai Medical College, delivered a welcome speech and announced that the Yunnan University of China (Yunnan)-Southeast Asian Cordyceps Biological Resources Sustainable Utilization International Joint Research Center conducted research, collection and specimen preparation for researchers at the Royal Thai Medical College of Thailand. Skills training projects such as strain isolation and artificial culture were opened.


Figure 2. Dr. Laliata Siriwattananon, Dean of the Thai Academy of Technology, Royal Thai Institute of Technology, welcomes you

On May 23, from 9:00 to 12:00, Prof. YU HONG gave a report titled "Cordyceps species in the world-the important species for economic impact", introducing more than 600 species of Cordyceps, Cloud Herb Labs worldwide. After 20 years of investigation and research, more than 450 species of Cordyceps species have been collected, and more than 8,500 strains have been deposited, with more than 18,000 specimens. Prof. Qi introduced the investigation and research work of Cordyceps sinensis, and introduced the Cordyceps species with important economic or medicinal value. It believes that Thailand has abundant Cordyceps biological resources and more than 350 species of Cordyceps, for Royal Institute of Technology. Investigating, collecting and researching more Cordyceps resources will help develop biomedical health products for the benefit of people in Southeast Asia.


Figure 3. Professor YU HONG's academic report at the Royal Institute of Technology

On May 23, 13:00-15:00, Professor YU HONG gave a report entitled "How to identify the Cordyceps species-the economically important cordycipitoid fungi", introducing the distribution of Cordyceps worldwide, species identification, species of Cordyceps, Cordyceps collection, isolation, preservation and artificial cultivation of Cordyceps species, focusing on methods for identifying cordyceps species based on morphological and molecular phylogenetic analysis, and conducting academic exchanges with trainers of the Royal Thai Polytechnic University.


Figure 4. Professor YU HONG conducts academic exchanges with trainers from the Royal Thai Polytechnic University

On May 23, 15:00-17:00, Dr. Wang Dong made a report entitled "Principle of technique of isolation and cultivation Cordyceps senso lato", introducing Cordyceps sample collection, specimen preparation, strain isolation, culture and preservation methods. Let the trainers of Thai Medical College have a preliminary understanding of the field investigation of Cordyceps and the construction of the germplasm bank of Cordyceps biological resources.


Figure 5. Dr. Wang Dong's academic report at the Royal Institute of Technology


Figure 6. YU HONG's Cordyceps research team and the Royal Institute of Technology researchers academic exchange photo

2. Training on the preparation of Cordyceps culture medium, separation, preservation and culture of Cordyceps

On May 24th, 9:00-17:00, Professor YU HONG's Cordyceps research team trained the researchers at the Royal Thai Polytechnic University on Cordyceps cultivation and investigation, including: preparation of the medium, isolation of Cordyceps species, preservation of Cordyceps species, Cordyceps Artificial culture and other technologies. First, Dr. Wang Dong explained and demonstrated the experimental operation, and then each trainer practiced. Professor YU HONG and Dr. Wang Dong gave on-the-spot guidance to the trainers until the trainers mastered the essentials.


Figure 7. Researchers at Royal Institute of Technology prepare experimental materials for Cordyceps species preservation


Figure 8. Royal Institute of Technology researchers prepare experimental equipment


Figure 9. Royal Institute of Technology experimenter preparation medium


Figure 10. Professor Yan teaches RMIT researchers experimental operation

3. Field investigation training of Cordyceps militaris biological resources

On May 25th, YU HONG, Professor of Yunnan University, and the Thai Academy of Sciences of Thailand, established a joint investigation team of 15 people, starting from Bangkok and driving more than 700 kilometers to the provincial capital of Chiang Rai, Chiang Rai, northern Thailand. Conduct field surveys of Cordyceps biological resources. The northeastern part of Chiang Rai and the north are bordered by Myanmar and Laos. It is located at an altitude of 580 meters above sea level. The climate in Chiang Rai is the lowest temperature in October and the lowest temperature in March and May. The hot season, especially in late May, is hot in the rainy season from June to September, with an average annual temperature of 24.6 °C.

From May 26th to 27th, the Cordyceps Biological Resources Joint Investigation Team conducted a survey of Cordyceps biological resources in the forests of the Khun Tan area of Chiang Rai. The Khun Tan area is located in the eastern part of Chiang Rai. The terrain is high mountains, valley plains, and alluvial plains. The average annual rainfall is 1768mm, the annual average temperature is 24°C, and the sampling site is about 1085 meters above sea level. According to preliminary statistics, a total of 20 specimens were collected, of which Beauveria was the dominant species.


Figure 11. Cordyceps habitat in Khun Tan District, Chiang Rai, Thailand


Figure 12. Beauveria sp.
Host: Hymenoptera (an ant); Habitat: Born on the evergreen broad-leaved forest or under the leaf layer


Figure 13. Cordyceps sp. Cordyceps sp
Host: Lepidoptera; habitat: born on evergreen broad-leaved forest or under falling leaves


Figure 14. Investigation of Cordyceps in the Khun Tan District of Chiang Rai, Thailand

On May 27th, 20:00-24:00, the joint investigation team carried out the separation of strains from the collected Cordyceps, and the researchers of Royal Institute of Technology were further familiar with the methods of isolation and culture of the strains. The joint investigation team will further separate the strains. The species are identified to determine their taxonomic status.


Figure 15. Dr. Wang Dong demonstrates the separation experiment of Cordyceps species


Figure 16. Cordyceps collected from Khun Tan District, Chiang Rai, Thailand

Through this visit, China (Yunnan)-Southeast Asia Cordyceps Biological Resources Sustainable Utilization International Joint Research Center and Thailand Royal Institute of Technology have deepened the cooperative relationship. Through training, researchers at Royal Thai Polytechnic University have mastered the investigation of Cordyceps, separation and preservation of strains. , artificial cultivation and other skills, set up a joint investigation team to conduct a preliminary investigation of the biological resources of Cordyceps sinensis in Thailand. The two sides will further carry out in-depth investigation and research on Cordyceps biological resources in Thailand, providing scientific support for the research and sustainable use of Cordyceps biological resources.


Figure 17. Group photo of the investigators of the Cordyceps resources in Chiang Rai, Thailand