A Survey of the Cordyceps Biological Resources Survey conducted by the Cordyceps Research Team of Yunnan University in Weishan County

Time: 
Saturday, January 11, 2020 - 12:45

August 12-August 17, 2019, September 17-September 21, The International Joint Research Center for Sustainable Utilization of Cordyceps Bioresources in China and Southeast Asia), two times to investigate the Cordyceps biological resources in the Daishan Yi and Hui Autonomous County of Dali Bai Autonomous Prefecture, Yunnan Province, in order to understand the species diversity of Cordyceps in this area. The main members of the two surveys include Prof. Yan from the School of Life Sciences of Yunnan University, Associate Professor Wang Li from Shandong Agricultural University, Sun Tao from Ph.D., Yunnan University, Master of Souvanhnachit Sisommay from Laos, Masters Tang Dexiang, Zhao Zhiyuan and Wang Zhiqin, Yunnan Yunyou Biotechnology Co., Ltd. The company and Yunnan Shangongtang Chinese Medicine Biotechnology Co., Ltd. Yao Xueyu and Lu Hongli. The two researchers involved in the investigation were Zhang Guoli, senior engineer of the Forestry and Forestry Bureau of Daishan County, Zhang Zeng, a senior agronomist of the Agricultural and Rural Bureau, and Yao Jiaren, a physician. According to preliminary statistics, a total of more than 200 specimens of Cordyceps were collected, totaling about 2 families, 4 genera and 19 species.

Dali Bai Autonomous Prefecture is located in the west of central Yunnan Province, with an altitude of 2,090 meters. It is adjacent to Chuxiong Prefecture in the east, Pu'er City and Linyi City in the south, Baoshan City and Nujiang State in the west, and Lijiang City in the north. It spans between 98°52′~101°03′ east longitude and 24°41′~26°42′ north latitude. It patrolls the Bohai Sea, the west and the Dangcang Mountain. It governs Dali City and Xiangyun, Midu, Binchuan and Yongping. The three counties of Yunlong, Wuyuan, Heqing and Jianchuan, as well as the three minority autonomous counties of Qionglai, Lushan and Nanxun, are one of the earliest areas in the southwestern frontier of China. The Daishan Yi and Hui Autonomous County is located in the western part of Yunnan Province, in the south of the Dali Bai Autonomous Prefecture. The county seat is 391 kilometers east of the provincial capital Kunming and 53 kilometers north of the state capital Dali.

The northern part of Lushan County is connected to Dali City, the east is adjacent to Midu County, the south is adjacent to Nanxun and Fengqing County, and the west is bordered by Yan and Changning County. Lushan County is part of the south extension of the Yunling Hengduan Mountains. It is located in the northern part of Ailao Mountain and Wuliang Mountain, and faces Dang Cangshan. Valleys, basins, and mountains are distributed. The terrain is high in the north, low in the south, low in the east and low in the west. The altitude difference is very large, with the lowest elevation of the river valley at 1146 meters and the highest elevation at the mountain level of 3037 meters. Lushan County is divided into two major river systems, the Honghe River and the Lancang River. The western mountain range is the watershed. The Lancang River basin to the west of the mountain is the Lancang River system. The Xihe River basin to the east of the mountain is the Red River system. The main rivers in the territory include Xihe River, Minjiang River and Leqiu River. Daishan County is rich in biological resources, including more than 70 wild mammals, mainly including deer, bear, fox, hedgehog, pangolin, etc. There are more than 100 kinds of wild birds, mainly green peacock, golden pheasant, thrush, green dragonfly, etc. . There are more than 300 kinds of wild plants, mainly including Yunnan pine, Huashan pine, alpine oak, orchid, chrysanthemum and so on. Valuable herbs include medlar, gastrodia, and fine safflower; precious mushrooms include pine mushrooms, chicken mites, mushrooms, and fungus.

The team of Professor Qi went to the Dangshan Yizu Hui Autonomous County in Dali Bai Autonomous Prefecture to investigate the Cordyceps biological resources, which is helpful for understanding the Cordyceps biological resources in the area and promoting the conservation and sustainable use of Cordyceps. In addition, it is also of great significance for the sustainable development and utilization of Cordyceps biological resources in Daishan County.

According to preliminary statistics, more than 200 specimens of Cordyceps were collected and more than 50 strains were isolated. The morphological identification identified a total of about 2 families, 4 genera and 19 species, respectively, Ophiocordycipitaceae Ophiocordyceps, Cordycuritaceae Beauveria and Cordyceps, and the dominant species Cordycipitaceae. The host mainly has three species: Lepidoptera, Hymenoptera, and Coleoptera.

1 Cordyceps Ophiocordycipitaceae

1.1 Cordyceps genus Ophiocordyceps

1.1.1 Cordyceps Ophiocordyceps pseudoacicularis

Host: Lepidopteran

Habitat: Mixed forest of often broad-leaved trees, wild mountain tea, cherry trees, and shrubs are azaleas.

1.1.2 Plateau Cordyceps Ophiocordyceps highlandensis

Host: 鳃金龟幼虫

Habitat: mixed forest of Pinus armandi and broad-leaved trees.

1.1.3 Cordyceps cinerea Cordyceps nutans Pat

 

Host: 蝽蟓

Habitat: broad-leaved forest.

1.1.4 Ant Worm Cordyceps Ophiocordyceps formicarum

 

Host: Ant

Habitat: Yunnan pine and broad-leaved mixed forest

1.2 Cordyceps militaris 01 Ophiocordyceps sp.01

Host: Lepidopteran

Habitat: Mixed forest of often broad-leaved trees, wild mountain tea, cherry trees, and shrubs are azaleas.

1.3 Cordyceps militaris 02 Ophiocordyceps sp.02

Host: Coleoptera

Habitat: Mixed forest of often broad-leaved trees, wild mountain tea, cherry trees, and shrubs are azaleas.

1.4 Cordyceps militaris 03 Ophiocordyceps sp.03

Host: spider

Habitat: broad-leaved mixed forest, mainly small trees. .

2 Cordycepsaceae Cordycipitaceae

2.1 Cordyceps Cordyceps Fr.

2.1.1 Cordyceps Cordyceps militaris

 

Host: Lepidoptera

Habitat: mixed forest of Pinus armandi and broad-leaved trees.

2.1.2 Cordyceps sinensis Cordyceps tenuipes

Host: Lepidopteran

Habitat: Mixed forest of often broad-leaved trees, wild mountain tea, cherry trees, and shrubs are azaleas.

2.1.3 Sporozoites Cordyceps farinose

Host: Hymenoptera

Habitat: alpine jungle, vegetation is Huashan pine, small shrub mixed forest.

2.1.4 S. Cordyceps Cordyceps cateniobliqua

 

Host: Coleoptera

Habitat: alpine jungle, vegetation is Huashan pine, small shrub mixed forest.

2.1.5 Cordyceps sinensis Cordyceps brongniartii

Host: Lepidoptera

Habitat: Alpine jungle, with Yunnan pine, eucalyptus and Huashan pine as the main mixed forest.

2.1.6 Moth Cordyceps Cordyceps tricentri

 

Host: Mo Yan

Habitat: alpine jungle, Huashan pine and small arbor mixed forest.

2.2 Beauveria bassiana Beauveria

2.2.1 Beauveria pseudomonas

Host: Hymenoptera

Habitat: mixed forest of Pinus armandi and broad-leaved trees.

2.3 Samsoniella

2.3.1 Samsoniella sp.01

 

 

Host: Coleoptera

Habitat: alpine jungle, dominated by Pinus armandii, eucalyptus and perennial herbs

2.4 Cordyceps a 01 Cordyceps sp.01

 

Host: Lepidopteran

Habitat: Mixed forest of often broad-leaved trees, wild mountain tea, cherry trees, and shrubs are azaleas.

2.5 Cordyceps 02 Cordyceps sp.02

 

Host: Lepidopteran

Habitat: Alpine jungle, with Yunnan pine, eucalyptus and Huashan pine as the main mixed forest.

2.6 Cordyceps a 03 Cordyceps sp.03

Host: Lepidopteran

Habitat: alpine jungle, vegetation is Huashan pine, small shrub mixed forest.

2.7 Cordyceps a 04 Cordyceps sp.04

Host: Lepidoptera

Habitat: Mixed forest of often broad-leaved trees, wild mountain tea, cherry trees, and shrubs are azaleas.

Professor Yan’s research team conducted a first survey of Cordyceps biological resources in Daishan County, Dali, and found that the area was rich in Cordyceps biological resources. A total of 7 plots and 6 habitats were investigated, and 2 species, 4 genera and 19 species were obtained. Six species were found in Xiaoshanshan, accounting for 31.5% of the total species diversity; two species were found in the bird's road, accounting for 10.52% of the total species diversity; two species were found in Qibaoshan, accounting for 10.52% of the total species diversity; Two species were found in Heyuan, accounting for 10.52% of the total species diversity; two species were found in Weidong Forest Farm, accounting for 10.52% of the total species diversity; one species was found in Wumaolin Reservoir, accounting for 5.26% of the total species diversity; Zijin Village 1 Species, accounting for 5.26% of the total species diversity; and Beauveria bassiana found in 7 plots, the distribution rate in several plots reached 100%; Cordyceps militaris found in Hongheyuan and Weidong forest farms, distribution rate Up to 20.57%; the fine-footed Cordyceps was found in the bird road Xiongguan, Weidong forest, Hongheyuan, Wumaolin reservoir plots, the distribution rate reached 57.1%.

The survey shows that the Xiaoshan foot mountain sample in Daishan County has an ecological environment suitable for the growth of Cordyceps, and the biodiversity of Cordyceps species is significantly higher than other plots. Beauveria bassiana was found in 7 plots, indicating that the species had diverse habitats, and the plots of Hongheyuan and Weidong forest farms were relatively moist, and Cordyceps militaris was the dominant species in the two species. Through two surveys, preliminary understanding of the distribution of Cordyceps militaris resources provides first-hand valuable information for the subsequent conservation and sustainable use of Cordyceps biodiversity in this area.

 

Figure 1 Professor Zuo Wei on the left and Associate Professor Wang Li on the right to investigate Cordyceps resources in the wild.

Figure 2: Zhang Guoli engineer on the left and Dr. Sun Tao on the right to investigate the Cordyceps resources in the field.

Figure 3 is Dr. Yao Jiaren on the left and Mr. Yao Xueyu on the right to investigate Cordyceps resources in the wild.

Figure 4 Left is Tang Dexiang, Wang Zhiqin Master, right is Souvanhnachit Sisommay Master in the wild to investigate Cordyceps resources

 

Figure 5 Different habitats of Cordyceps sinensis in Daishan County

Figure 6 Team of Yunnan University professors surveyed Cordyceps in the Red River source and the chicken foot mountain

Figure 7 Team of professors from Yunnan University investigates Cordyceps in the wild in the bird road and in the wilderness of the mountain

 

Figure 8 Team of professors from Yunnan University investigates Cordyceps in the wild in Wumaolin Reservoir and Weidong Forest Farm

Figure 9 Team of professors from Yunnan University investigates Cordyceps in the wild

 

Table 1 Collection information

Look like

longitude(E)

latitude(N)

altitude(m)

Chicken foot mountain

100.19°

25.14°

2705±10

Qi Baoshan

100.34°

25.18°

2255±10

Bird Road

100.34°

25.29°

2476±10

Zijin Village

100.11°

25.37°

2752±10

Hongheyuan

100.12°

25.49°

2267±10

Weidong Forest Farm

100.36°

25.15°

2205±10

Wumaolin Reservoir

100.52°

25.10°

1850±10

 

Professor Yan’s research team went to Lushan to investigate the Cordyceps resources and coincided with the Zhongyuan Festival. The people of Lushan Mountain were very simple and enthusiastic to invite us to experience the customs and customs of Lushan Mountain. When you were busy, Professor Yan wrote and wrote. Two words to commemorate the trip to Lushan.

                                

 

 

 

Thanks

The research team of Professor Yan of Yunnan University went to Dangshan County of Dali to investigate the Cordyceps resources, and thanked the People's Government of Daishan County for their support in the work. Especially, the county magistrate Zhou Xiaofang and the deputy magistrate Li Xuegang gave great strength in the two investigations. Support; I am grateful to the Director of Agriculture and Rural Bureau of Daishan County, Mr. Zhou, Director of the Agricultural and Rural Bureau, Zhang Zeng, Senior Engineer of the County Forest and Grass Bureau, Zhang Guoli and Huang Xuezhi for participating and helping in the investigation; thanking Yao Jiaren, Dr. Zhang Hui, etc. People's hard participation in the investigation; especially thanks to Mr. Chen Jiawei, the general manager of Nanxun Ancient Capital Tourism Development Co., Ltd., to provide us with strong logistical support, so that our two investigations went smoothly; thanks to Professor Wang Li of Shandong Agricultural University, who participated in the investigation of Cordyceps resources. Thanks to Yunnan Yunyou Biotechnology Co., Ltd. and Yunnan Shangongtang Traditional Chinese Medicine Biotechnology Co., Ltd. Mr. Yao Xueqi and Ms. Lu Hongli for their direction, transportation support and hard participation during the whole research process; thanks to the two masters such as Sun Rulong and Li Fengwei Transportation support; finally thank you again for the People’s Government of Lushan Support and help, so that the two surveys rewarding.